Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3

double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show that Saccharomyces cerevisiae Sgs1 and Top3 proteins are sufficient to migrate and disentangle a dHJ to produce exclusively non-crossover recombination products, in a reaction termed "dissolution." We show that Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 protein concentrations, although it has no effect on the initial rate of Holliday junction (HJ) migration. Rmi1 serves to stimulate DNA decatenation, removing the last linkages between the repaired and template DNA molecules. Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity

The impact of the AuScope VLBI observations and the regional AUSTRAL sessions on the TRF

The AuScope VLBI array was built with the purpose to improve the terrestrial (TRF) and celestial reference frames in the southern hemisphere. Since 2010 the three 12-m antennas in Hobart (Tasmania), Katherine (Northern Territory) and Yarragadee (Western Australia) heavily contribute to the global VLBI observations coordinated by the International VLBI Service for Geodesy and Astrometry. In 2011, the AUSTRAL VLBI program was started, with more than 40 sessions being observed so far. In the AUSTRALs, the three AuScope antennas observe together with the new 15-m dish in Hartebeesthoek (South Africa) and the 12-m antenna in Warkworth (New Zealand). Recently, the planned observations have been expanded again, with 50 additional sessions scheduled until mid-2015, along with 3 continuous campaigns covering 15 days each. All AUSTRALs are recorded with an increased data rate of 1 Gbps, allowing to compensate for the reduced sensitivity of the generally smaller dish size. We evaluate the positive impact of the AuScope VLBI program on the global TRF. This is due to the increased number of observations and the improved homogeneity of the global VLBI network. All data collected within this intense observing program is analysed and geodetic results are presented. This includes time series of baseline lengths and station coordinates of the contributing stations. We compare the results obtained within the regional AUSTRAL sessions with the ones of the classical global VLBI networks and identify superiorities and shortcomings of both. The high number of sessions gives high accuracies and good repeatabilities of the determined parameters. Additionally, remaining variations of baseline lengths can be identified and are compared against by default un-modelled station motions due to hydrology and atmosphere loading. Finally, we give an outlook on future plans for the AuScope antennas and the AUSTRAL observing program: on future operations, expected improvements through hardware upgrades as well as research on the use of sibling telescopes available at two sites within the AUSTRAL array (Hobart and Hartebeesthoek)

The Paths People Take Through Teaching Center Services: A Descriptive Analysis

Teaching centers offer a variety of services, ranging from teaching orientations and one-time workshops to intensive programs such as learning communities to individual consultations. However, most instructors do not participate in all categories of service a center offers; rather, they create their own paths through various combinations and sequences of programs. What do we know about these pathways, and what can we learn from the patterns of use? This article shares findings from an analysis of several years of data to learn more about the sequence in which instructors experience educational development and to discuss the implications of these findings

The mechanics of inelastic buckling using a Shanley-like model

This paper presents a study of the mechanics of inelastic buckling using a Shanley-like simplified column model. The model is an extension of the original Shanley model with multiple springs and two dampers. The inclusion of damping enables the dynamic response of the model under constant loading to be captured. The model has been evaluated against the tangent-modulus and reduced-modulus critical buckling loads, and has been found effective in representing the progressive change in the regions of loading and unloading during inelastic buckling. It is also able to simulate the extreme situations of inelastic buckling by varying the ratio of the two damping coefficients. It is seen that high rotational damping, relative to vertical damping, causes the buckling to move towards the reducedmodulus buckling load at much lower deflections than when the relationship is reversed

Using InSAR stacking techniques to predict bridge collapse due to scour

Failure of bridges due to scour is of great concern to bridge asset owners, and is currently very difficult to predict and monitor regularly using conventional assessment methods. This paper presents evidence of how InSAR techniques can be used to monitor bridges at risk of scour, using Tadcaster Bridge, England, as a case study. Tadcaster Bridge suffered a partial collapse due to river scour on the evening of December 29th, 2015 following a period of severe rainfall and flooding. SAR scenes over the bridge from the two-year period prior to the collapse are analysed using SBAS interferometry methods, highlighting a distinct movement in the region of the bridge where the collapse occurred prior to the actual event. This precursor to failure observed in the data suggests the possible use of InSAR in structural health monitoring of bridges at risk of scour, as a means of an early warning system